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Free light-chain proteinuria and
normal renal histopathology and function in 11 dogs exposed to
Lleishmania infantum, Ehrlichia canis, and Bbabesia
canis.
Bonfanti U, Zini E, Minetti E, Zatelli
A.
Clinica Veterinaria Gran Sasso, Milano, Italy.
u.bonfa@flashnet.it
The purpose of this retrospective study was
to investigate the relationship among proteinuria consisting of
immunoglobulin free light chains (FLCs), renal histopathologic findings,
and routine markers of renal function in 11 dogs exposed to Leishmania
infantum (n = 8), Ehrlichia canis (n = 2), and Babesia canis (n = 1).
FLC proteinuria was suspected based on identification of a 22- to 27-kDa
band by sodium dodecyl sulfate-agarose gel electrophoresis (SDS-AGE) and
later confirmed by immunofixation electrophoresis. SDS-AGE identified an
isolated band of 22-27 kDa in 8 dogs, whereas the remaining 3 had a 22-
to 27-kDa band and an additional band of 67-72 kDa. The median urine
protein-to-urine creatinine ratio was 0.37 (range, 0.11-2.24) and
increased ratios were found in 6 dogs (54.5%) (reference value,
<0.7). All dogs underwent histologic examination of renal
percutaneous biopsy specimens and determination of serum creatinine and
urea concentrations. Tissue samples for light microscopy were stained
with hematoxylin-eosin, periodic acid-Schiff, Goldners trichrome, and
methenamine silver. In the study group, the glomerular tufts, mesangium,
tubulointerstitium, and vessels appeared unaffected. The median serum
creatinine concentration in these 11 dogs was 1.3 mg/dL (range, 0.8-1.5
mg/dL; reference range, 0.6-1.5 mg/dL), whereas the concentration for
urea was 28 mg/dL (range, 22-52 mg/dL; reference range, 20-50 mg/dL).
All dogs had normal renal morphology and had normal serum creatinine and
urea concentrations, suggesting that immunoglobulin FLC may be detected
in the urine of dogs exposed to L. infantum, E. canis, and B. canis
without any apparent structural or functional renal
derangement.
PMID: 15515575 [PubMed - in process]
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